Molecular genotyping of methicillin-resistant Staphylococcus aureus via fluorophore-enhanced repetitive-sequence PCR
نویسندگان
چکیده
منابع مشابه
Molecular epidemiology of methicillin-resistant Staphylococcus aureus.
Subtyping methicillin- resistant Staphylococcus aureus (MRSA) isolates and tracking nosocomial infections have evolved from phenotypic to genotypic approaches; most laboratories now depend on pulsed-field gel electrophoresis (PFGE). We discuss the limitations of current image-based genotyping methods, including PFGE, and the advantages (including ease of entering data into a database) of using ...
متن کاملBlinded comparison of repetitive-sequence PCR and multilocus sequence typing for genotyping methicillin-resistant Staphylococcus aureus isolates from a children's hospital in St. Louis, Missouri.
We performed a blinded study to compare repetitive-sequence PCR and multilocus sequence typing for genotyping hospital- and community-acquired methicillin-resistant Staphylococcus aureus (MRSA). The MRSA strains that were sequence type 8 (ST8), staphylococcal cassette chromosome mec (SCCmec) type IV, and Panton-Valentine leukocidin-positive clustered separately from those that were ST5 and SCCm...
متن کاملMethicillin Resistant Staphylococcus aureus
Etiology Staphylococcus aureus is a Gram positive, coagulase positive coccus in the family Staphylococcaceae. Methicillin-resistant S. aureus (MRSA) strains are resistant to methicillin and essentially all other beta-lactam antibiotics. MRSA isolates are genetically heterogeneous. 1 Some strains, which are called epidemic strains; are more prevalent and can spread within or between hospitals an...
متن کاملMethicillin Resistant Staphylococcus aureus
Staphylococcus aureus is an opportunistic pathogen often carried asymptomatically on the human body. Methicillin-resistant S. aureus (MRSA) strains have acquired a gene that makes them resistant to all beta-lactam antibiotics. Hospital-associated strains of this organism are serious nosocomial pathogens that have become resistant to most common antibiotics, and treatment can be challenging. Com...
متن کاملMethicillin-resistant Staphylococcus aureus
received much attention over the past 30 years because of its increasing incidence and the morbidity and excess hospital stay length associated with MRSA infection. Th e current controversy about the eff ectiveness of control measures, notably screening, derives from the confl icting results of available studies, lack of well-designed controlled studies, and concomitant use of several intervent...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1995
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.33.8.2141-2144.1995